Journal:

Article Title: Use of a Recombinant Fluorescent Substrate with Cleavage Sites for All Botulinum Neurotoxins in High-Throughput Screening of Natural Product Extracts for Inhibitors of Serotypes A, B, and E ▿ †

doi: 10.1128/AEM.01690-07

Figure Lengend Snippet: Inhibition of BoNT A protease activity in HPLC assays by selected extracts from plate 96110120

Article Snippet: Compounds from extracts were purified by reverse-phase HPLC on a 0.5- by 25-cm C 18 column (Hi-Pore RP-318; Bio-Rad, Richmond, CA) using gradients of acetonitrile in 0.1% trifluoroacetic acid.

Techniques: Inhibition, Activity Assay, Control

Journal:

Article Title: Use of a Recombinant Fluorescent Substrate with Cleavage Sites for All Botulinum Neurotoxins in High-Throughput Screening of Natural Product Extracts for Inhibitors of Serotypes A, B, and E ▿ †

doi: 10.1128/AEM.01690-07

Figure Lengend Snippet: Extracts that inhibited at least 30% in HPLC assays

Article Snippet: Compounds from extracts were purified by reverse-phase HPLC on a 0.5- by 25-cm C 18 column (Hi-Pore RP-318; Bio-Rad, Richmond, CA) using gradients of acetonitrile in 0.1% trifluoroacetic acid.

Techniques:

Journal:

Article Title: Use of a Recombinant Fluorescent Substrate with Cleavage Sites for All Botulinum Neurotoxins in High-Throughput Screening of Natural Product Extracts for Inhibitors of Serotypes A, B, and E ▿ †

doi: 10.1128/AEM.01690-07

Figure Lengend Snippet: Fractionation of Terminalia brownii root bark extract by reverse-phase HPLC. (A) Chromatogram of the column effluent monitored at 210 nm. Peaks 1 and 2 inhibited BoNT A protease activity. (B) Absorbance spectra of peaks 1 and 2. (C) Absorbance spectrum of myricetin.

Article Snippet: Compounds from extracts were purified by reverse-phase HPLC on a 0.5- by 25-cm C 18 column (Hi-Pore RP-318; Bio-Rad, Richmond, CA) using gradients of acetonitrile in 0.1% trifluoroacetic acid.

Techniques: Fractionation, Activity Assay